SPR可以進(jìn)行藥物靶量測(cè)量，從藥物-膜的相互作用，到藥物-細(xì)胞的相互作用。在實(shí)時(shí)和無(wú)標(biāo)記的情況下區(qū)分內(nèi)部和滲透。研究細(xì)胞在不同涂層的附著情況。
基于細(xì)胞的研究有很多，包括組織工程、體外毒物學(xué)、藥物輸送，還有抗菌涂料。表面等離子體共振最初是用于蛋白質(zhì)相互作用測(cè)量的。直到近年來，研究人員才開始開發(fā)SPR來研究活細(xì)胞。
我們的一些用戶使用SPR來測(cè)量細(xì)胞在不同的生物材料和涂層表面的擴(kuò)散或粘附。
SPR也可作為生物傳感器用于檢測(cè)細(xì)胞特定代謝物以確定細(xì)胞分化和細(xì)胞功能
一些小組正在研究生物膜的形成或抗菌涂料
五個(gè)選擇SPR檢查活細(xì)胞的原因
1. 能夠?qū)崟r(shí)測(cè)量活細(xì)胞和細(xì)菌附著
2. 能夠在未標(biāo)記的情況下區(qū)分滲透和內(nèi)吞
3. 表面非特異性吸附情況
4. 溫度和剪切應(yīng)力的控制
5. SPR與電化學(xué)的結(jié)合
SPR可以回答的關(guān)于活細(xì)胞的五個(gè)關(guān)鍵性問題
1. 藥物吸收途徑是什么?
2. 哪一種納米顆粒是藥物輸送的最佳載體?
3. 一種納米粒子或病毒是如何進(jìn)入細(xì)胞的?
4. 細(xì)胞附著在表面上的動(dòng)力學(xué)是什么?
5. 哪一種表面最能抵抗細(xì)菌的生長(zhǎng)?
參考文獻(xiàn)
1. Endothelial cells' biophysical, biochemical, and chromosomal aberrancies in high ‐ glucose condition within the diabetic range, Cell Biochemistry & Function, 2017, p. 1-15
2. Feasibility Study of the Permeability and Uptake of Mesoporous Silica Nanoparticles across the Blood-Brain Barrier, PLoS ONE 11(8): e0160705, 2016
3. Real-Time Label-Free Monitoring of Nanoparticle Cell Uptake, Small, 2016
4. Real-Time Protein and Cell Binding Measurements on Hydroxyapatite Coatings, J. of Functional Biomaterials, 2016, Vol. 7(3), p. 23
5. Biomimetic collagen I and IV double layer Langmuir–Schaefer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells, Biomaterials, Volume 1, May 2015, Pages 257-269
6. Structural and Viscoelastic Properties of Layer-by-Layer Extracellular Matrix (ECM) Nanofilms and Their Interactions with Living Cell, ACS Biomater. Sci. Engineering, 2015, 1[9]: p. 816–824
7. Microbial attachment and adsorption–desorption kinetic of tightly bound extracellular polymeric substances on model organic surfaces, Chemical Engineering Journal, 2015, 279: p. 516–521
8. Investigation of cell behaviors on thermo-responsive PNIPAM microgel films, Colloids and Surfaces B: Biointerfaces, 132: p. 202–207
9. Biofunctionalization of titanium surface with multilayer films modified by heparin-VEGF-fibronectin complex to improve endothelial cell proliferation and blood compatibility, J. of Biomed. Mat. Research Part A, 2013, 101A, 413–420
10. Oncolytic adenoviruses coated with MHC-I tumor epitopes increase the anti-tumor immunity and efficacy against melanoma, Capasso et al., Taylor & Francis Online, 2015
